Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Porcine fungal mock community analyses: Implications for mycobiome investigations

doi: 10.3389/fcimb.2023.928353

Figure Lengend Snippet: Composition of fungal genomic DNA found in ATCC MSA 1010 mix (ATCC Reference Standard).

Article Snippet: 10% , Penicillium chrysogenum (ATCC 10106D-5).

Techniques:

Journal:

Article Title: The C-Terminal Region of Bacteroides fragilis Toxin Is Essential to Its Biological Activity

doi: 10.1128/IAI.00135-06

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Antibiotics (Sigma, St. Louis, MO) were used at the following concentrations: for Escherichia coli , 150 μg/ml ampicillin and 1 μg/ml ciprofloxacin; and for B. fragilis , 6 μg/ml clindamycin. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant genotype, phenotype, and/or characteristic(s) a Source and/or reference Strains 86-5443-2-2 ETBF strain L. L. Myers NCTC 9343 NTBF strain ATCC ( 11 ) Plasmids pRK231 Tra + IncPα Ap r 10 pFD340 E. coli/B. fragilis shuttle vector, Ap r Cc r , IS 4351 promoter 32 pFD340::P- bft pFD340 containing bft plus 700-bp upstream region 7 pMA17 BFT C-terminal −2 truncation mutant in pFD340::P- bft This study pMA11 BFT C-terminal −4 truncation mutant in pFD340::P- bft This study pMA12 BFT C-terminal −7 truncation mutant in pFD340::P- bft This study pMA13 BFT C-terminal −8 truncation mutant in pFD340::P- bft This study pMA14 BFT C-terminal −9 truncation mutant in pFD340::P- bft This study pMA15 BFT C-terminal −11 truncation mutant in pFD340::P- bft This study pMA16 BFT C-terminal −18 truncation mutant in pFD340::P- bft This study pMA18 Replacement of BFT-A394 with E in pFD340::P- bft This study Open in a separate window a Ap r , ampicillin resistance; Cc r , clindamycin resistance.

Techniques: Plasmid Preparation, Mutagenesis

Journal: Microbial Biotechnology

Article Title: Rio Tinto as a niche for acidophilus enzymes of industrial relevance

doi: 10.1111/1751-7915.14192

Figure Lengend Snippet: Graphical representation of the differences in glucose (up) and xylose (down) released in pretreated corn stover (PCS) and pretreated sugarcane straw (PSCS) in hydrolysis when the secretome of M. thermophila C1‐derived cocktail was supplemented with 1 mg g −1 of Rio Tinto secretomes. The M. termophila enzymatic cocktail allowed the release of glucose (≈45 g kg −1 ) and xylose (≈14.5 g kg −1 ).

Article Snippet: Rio Tinto, with its extraordinary environmental conditions, is an excellent niche for acidophilus microorganisms and we have demonstrated that enzymatic cocktails derived from, at least, 26% of the Rio Tinto collection strains (50% of the strains assayed in hydrolysis) improved the released of sugars from industrial substrates and therefore are suitable to be used for 2G technology.

Techniques: Derivative Assay

Journal: PLoS ONE

Article Title: An N-Myristoylated Globin with a Redox-Sensing Function That Regulates the Defecation Cycle in Caenorhabditis elegans

doi: 10.1371/journal.pone.0048768

Figure Lengend Snippet: A ) Defecation cycle under normal conditions does not significantly differ between the wild type N2 and glb-26(tm4837) . When exposed to 10 mM PQ, the wild type shows a strong slowing down of the defecation cycle, whereas this slowing down is weaker in glb-26(tm4837) . Error bars indicate standard error (n = 3), * p<0.05; *** p<0.001. B ) Survival of N2 and glb-26 (tm4837) on 10 mM PQ does not significantly differ. Error bars indicate standard error (n = 3).

Article Snippet: The glb-26 (tm4837) knockout strain was provided by the Japanese National Bioresource Project. glb-26 (tm4837) was outcrossed eight times with N2 to remove adventitious mutations.

Techniques:

Journal: BMC Genomics

Article Title: Comparative genomic analysis of pathogenic factors of Listeria spp. using whole-genome sequencing

doi: 10.1186/s12864-024-10849-3

Figure Lengend Snippet: Maximum likelihood phylogeny of 29 Listeria strains and 1 strain of S. aureus used as the outgroup based on whole-genome sequences. The four Listeria strains used in this study are indicated in red boxes, and white indicates unknown information. a: year of sample collection, b: sample isolation source, c: geographic origin, d: serotype

Article Snippet: Subsequently, cluster analysis was conducted on the WGS of 26 reference strains of Listeria spp. sourced from the National Center for Biotechnology Information and 4 isolates from this study (Fig. ).

Techniques: Isolation